BAC PROJECT
16 | monthsFUNDAIRIES

Development of functional goat and sheep dairies with added native strains and olive leaf polyphenols

Related toSpoke 04

Principal investigators
Monica Deiana

Other partecipants G. Serreli, S. Cosentino, B. Pisano, C.I.G. Tuberoso, M. Budroni, G. Zara, A. Bianco, M. C. Cossu, M. Addis, M. Pes, C. Piga, R. Comunian, M. Campus, A. Argiolas, V. Tomasiello, E. Ortu, F. Congiu, E. Falchi, N. Baldussu, A. Boi, F. Lorrai, A. Deiana, P. Buratti, M. Cabras
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Project partners

Università degli Studi di Cagliari

Coordinator

Other partners

Università degli Studi di Sassari, Agenzia AGRIS Sardegna, Caseificio Silvio Boi S.r.l, Argiolas Formaggi S.r.l

Task involved

Task 4.1.1.

Reformulation or improvement of relevant food products in the national context in order to: a) implement the nutritional characteristics also by reducing antinutrients or using bioprocessed ingredients (from raw products to ingredients) and limit the use of undesirable components (e.g. by using bioactives) along the food system b) improve food formulation and composition based on consumer perception and needs identified for specific target groups (in connection with Spoke 5 and 6) c) promote clear and ad hoc labelling as an information tool (in connection with Spoke 1 and 7) to increase the willingness to buy improved foods d) guarantee safety and affordability of new products (in connection with Spoke 1 and 3).

Task 4.1.2.

Innovation of food (bio)processing using smart and mild technologies and fermentation to improve nutritional quality while ensuring safety and environmental sustainability throughout the shelf life of foods. Nutritional quality and biodiversity are targeted through both advanced and sustainable processes (including encapsulation) to preserve and improve at-risk (micro)nutrient composition of relevant food categories and exploiting microbiological and biotechnological applications to impact on nutritional quality. Such (bio)technological approaches (e.g., fermentation, enzyme treatments, etc.) are validated by process markers also directed to ensure food production safety and quality targeting new food habits (e.g., ready to eat food and novel food consumption) and sustainability, promoting production efficiency and utilisation of alternative sources (in connection with Spoke 2 and 3).

State of the art

Reformulation of foods with polyphenol rich fractions recovered from agri-food by-products is considered the best method to meet the needs of today’s consumers, in line with the concept of circular economy. 

Polyphenols have been incorporated into several food products with the aim to increase polyphenol intake, and thus their healthy properties, and to improve shelf life and prevent food spoilage. The phenolic extract made from olive leaves, the most abundant by-product of the olive grove industry, OLE, has recently being used in food productions as functional ingredient, since it has high amounts of bioactive phenolics, such as oleuropein and hydroxytyrosol. 

OLE has also been shown to exert prebiotic properties and to interact with the microbiota of fermented foods, like yogurts and cheeses. So far, however, only few experimental fortifications with OLE were proposed for dairy products, an excellent matrix for the release of added bioactive compounds. 

The project fits into this context, aiming to develop new functional dairies starting from the typical local productions of Sardinia from sheep and goat milk, added with OLE, dietary fibres, commercial probiotic bacteria and native microbial strains with potential functional properties.

Operation plan

The project has been developed according to a step-by-step procedure carried out by a multidisciplinary team. The operational plan included different activities as reported below:

  1. Collection of the olive leaves and phenolic extract (OLE) production
  2. Chemical and functional characterization, in terms of antioxidant and anti-inflammatory properties, of OLE
  3. Analysis of OLE effect on in vitro properties related to the functionalities of starters and commercial or native potentially probiotic strains
  4. Pilot scale production and first evaluation of 3 new dairy products from sheep/goat milk added with OLE, commercial and native starters, dietary fibres, and commercial/native potentially probiotic bacteria
  5. Evaluation of physico-chemical, nutritional, microbiological and sensory features, overall antioxidant activity and shelf-life of the prototype dairy products, together with the quali-quantitative analysis of the phenolic components derived from the fortification with OLE in the products
  6. Study of the adaptability of pilot production processes and technological transfer within the context of the dairy companies
  7. Evaluation of the shelf-life of final dairy products, growth and survival of the inoculated microbial strains (starters and probiotics), and formulation of the nutritional label

Results achieved

The development of the project led to the successful achievement of the proposed objective, the production of OLE-fortified sheep’s and goat’s milk dairy products at industrial scale. We developed 3 new dairy products with predefined functional features and specific nutritional claims:

  • Fermented Milk: from sheep’s milk, high fibre content, high protein content, lactose-free, fortified with OLE – Caseificio Silvio Boi
  • Casu Axedu (Traditional Agrifood Product, TAP, of Sardinia): from sheep's milk, source of fibre, high protein content, lactose-free, fortified with OLE, added with probiotics – Caseificio Silvio Boi
  • Fresh Goat Cheese: from goat's milk, source of fibre, high protein content, lactose-free, fortified with OLE, added with probiotics – Caseificio Argiolas formaggi

Both experimental and scaled-up batches met the set targets for physico-chemical, nutritional, microbiological and sensory attributes, confirming process reproducibility and the effectiveness of the adaptations implemented at each dairy plant.

All products showed remarkable stability in pH, macronutrient, and functional ingredient composition throughout the storage period, aligning with EU regulatory demands for nutritional and health claims.